至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

A pH-engineering regenerative DNA tetrahedron ECL biosensor for the assay of SARS-CoV-2 RdRp gene based on CRISPR/Cas12a -activity

Chem Eng J. 2021-09; 
Kai Zhang, Zhenqiang Fan, Yuedi Ding, Minhao Xie
Products/Services Used Details Operation
Custom DNA/RNA Oligos … The oligonucleotides purified by high-performance liquid chromatography (HPLC) used in our detection strategy were purchased from Genscript Biotech (Nanjing, China). Gold chloride trihydrate (HAuCl 4 ·3H 2 O), sodium citrate, potassium persulfate (K 2 S 2 O 8 ), and sodium … Get A Quote

摘要

In this work, we constructed an exonuclease III cleavage reaction-based isothermal amplification of nucleic acids with CRISPR/Cas12a-mediated pH-induced regenerative Electrochemiluminescence (ECL) biosensor for ultrasensitive and specific detection of SARS-CoV-2 nucleic acids for SARS-CoV-2 diagnosis. The triple-stranded nucleic acid in this biosensor has an extreme dependence on pH, which makes our constructed biosensor reproducible. This is essential for effective large-scale screening of SARS-CoV-2 in areas where resources are currently relatively scarce. Using this pH-induced regenerative biosensor, we detected the SARS-CoV-2 RdRp gene with a detection limit of 43.70 aM. In addition, the detection system ha... More

关键词

CRISPR/Cas12a, DNA tetrahedron, ECL, Regenerative Biosensor, SARS-CoV-2 RdRp gene, Triple-helix