Products/Services Used | Details | Operation |
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Custom DNA/RNA Oligos> | … The oligonucleotides purified by high-performance liquid chromatography (HPLC) used in our detection strategy were purchased from Genscript Biotech (Nanjing, China). Gold chloride trihydrate (HAuCl 4 ·3H 2 O), sodium citrate, potassium persulfate (K 2 S 2 O 8 ), and sodium … | Get A Quote |
In this work, we constructed an exonuclease III cleavage reaction-based isothermal amplification of nucleic acids with CRISPR/Cas12a-mediated pH-induced regenerative Electrochemiluminescence (ECL) biosensor for ultrasensitive and specific detection of SARS-CoV-2 nucleic acids for SARS-CoV-2 diagnosis. The triple-stranded nucleic acid in this biosensor has an extreme dependence on pH, which makes our constructed biosensor reproducible. This is essential for effective large-scale screening of SARS-CoV-2 in areas where resources are currently relatively scarce. Using this pH-induced regenerative biosensor, we detected the SARS-CoV-2 RdRp gene with a detection limit of 43.70 aM. In addition, the detection system ha... More