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Bacterial Expression> | … thaliana HMGR2 (AtHMGR2, AY143930.1) were synthesized by GenScript Inc. (Nanjing, China) to remove the internal BsaI or BpiI recognition sites; and a truncated version of AtHMGR2 without the 99 N-terminal amino acids was used. E. coli UbiC (NC_000913.3) was also … | Get A Quote |
Coenzyme Q (CoQ) is vital for energy metabolism in living organisms. In humans, CoQ deficiency causes diseases and must be replenished via diet; however, CoQ content in plant foods is primarily low. Here, we report the breeding of high CoQ tomato lines by expressing four enzymes with a fruit-specific promoter, which modifies the chloroplast chorismate pathway, enhances cytosolic isoprenoid biosynthesis, and up-regulates the first two reactions in mitochondrion that construct the CoQ polyisoprenoid tail. We show that, while the level of the aromatic precursor could be markedly elevated, head group prenylation is the key to increasing the final CoQ yield. In the HUCD lines expressing all four transgenes, the high... More