Products/Services Used | Details | Operation |
---|---|---|
Custom DNA/RNA Oligos> | … have contributed equally to this work. This article was submitted to Parasite Immunology, a section of the journal Frontiers in Immunology … endotoxin levels of the recombinant protein were detected using a Toxin Sensor™ Gel Clot Endotoxin Assay Kit (Genscript, {"type":"entrez … | Get A Quote |
Macrophages promote early host responses to infection by releasing pro-inflammatory cytokines, and they are crucial to combat amoebiasis, a disease affecting millions of people worldwide. Macrophages elicit pro-inflammatory responses following direct cell/cell interaction of , inducing NLRP3 inflammasome activation with high-output IL-1β/IL-18 secretion. Here, we found that trophozoites could upregulate peroxiredoxins (Prx) expression and abundantly secrete Prxs when encountering host cells. The C-terminal of Prx was identified as the key functional domain in promoting NLRP3 inflammasome activation, and a recombinant C-terminal domain could act directly on macrophage. The Prxs derived from triggered toll-like... More