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A Novel TLR4-Binding Domain of Peroxiredoxin From Triggers NLRP3 Inflammasome Activation in Macrophages

Front Immunol. 2021-09; 
Xia Li, Meng Feng, Yanqing Zhao, Yuhan Zhang, Ruixue Zhou, Hang Zhou, Zhen Pang, Hiroshi Tachibana, Xunjia Cheng
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Custom DNA/RNA Oligos … have contributed equally to this work. This article was submitted to Parasite Immunology, a section of the journal Frontiers in Immunology … endotoxin levels of the recombinant protein were detected using a Toxin Sensor™ Gel Clot Endotoxin Assay Kit (Genscript, {"type":"entrez … Get A Quote

摘要

Macrophages promote early host responses to infection by releasing pro-inflammatory cytokines, and they are crucial to combat amoebiasis, a disease affecting millions of people worldwide. Macrophages elicit pro-inflammatory responses following direct cell/cell interaction of , inducing NLRP3 inflammasome activation with high-output IL-1β/IL-18 secretion. Here, we found that trophozoites could upregulate peroxiredoxins (Prx) expression and abundantly secrete Prxs when encountering host cells. The C-terminal of Prx was identified as the key functional domain in promoting NLRP3 inflammasome activation, and a recombinant C-terminal domain could act directly on macrophage. The Prxs derived from triggered toll-like... More

关键词

Entamoeba histolytica, NLRP3 inflammasome, TLR4-binding domain, macrophage, peroxiredoxin