Products/Services Used | Details | Operation |
---|---|---|
Bacterial Expression> | … 2009). Codon optimized for E. coli expression, double mutant plasmid SULT1A1 K56ER68G in pET-19b was from GenScript (New Jersey, United States). Escherichia coli strain Bi 8337/41(O10:K5:H4) expressing K5 polysaccharide was from ATCC (Virginia, United States) … | Get A Quote |
N-Deacetylase/N-sulfotransferases (NDSTs) are critical enzymes in heparan sulfate (HS) biosynthesis. Radioactive labeling assays are the preferred methods to determine the N-sulfotransferase activity of NDST. In this study, we developed a fluorometric coupled enzyme assay that is suitable for the study of enzyme kinetics and inhibitory properties of drug candidates derived from a large-scale in silico screening targeting the sulfotransferase moiety of NDST1. The assay measures recombinant mouse NDST1 (mNDST1) sulfotransferase activity by employing its natural substrate adenosine 3'-phophoadenosine-5'-phosphosulfate (PAPS), a bacterial analog of desulphated human HS, Escherichia coli K5 capsular polysaccharide (... More