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The component of the mA writer complex VIRMA is implicated in aggressive tumor phenotype, DNA damage response and cisplatin resistance in germ cell tumors

J Exp Clin Cancer Res. 2021-08; 
Vera Miranda-Gonçalves, João Lobo, Catarina Guimarães-Teixeira, Daniela Barros-Silva, Rita Guimarães, Mariana Cantante, Isaac Braga, Joaquina Maurício, Christoph Oing, Friedemann Honecker, Daniel Nettersheim, Leendert H J Looijenga, Rui Henrique, Carmen Jerónimo
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Synthetic sgRNA and crRNA Service … to cisplatin compared to 2102Ep and NT2, as documented in our previous study [26]) was chosen to perform VIRMA knockdown by plasmids carrying the CRISPR/Cas9 system containing a guide RNA sequence (available in [29]) targeting this gene (obtained from GenScript, … Get A Quote

摘要

background: Germ cell tumors (GCTs) are developmental cancers, tightly linked to embryogenesis and germ cell development. The recent and expanding field of RNA modifications is being increasingly implicated in such molecular events, as well as in tumor progression and resistance to therapy, but still rarely explored in GCTs. In this work, and as a follow-up of our recent study on this topic in TGCT tissue samples, we aim to investigate the role of N6-methyladenosine (mA), the most abundant of such modifications in mRNA, in in vitro and in vivo models representative of such tumors. methods: Four cell lines representative of GCTs (three testicular and one mediastinal), including an isogenic cisplatin resistant su... More

关键词

CAM, CRISPR/Cas9, Cisplatin, DNA repair, Epitranscriptomics, Germ cell tumors, N6-methyladenosine, RNA modifications, VIRMA