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CD4+ T cells in the lungs of acute sarcoidosis patients recognize an Aspergillus nidulans epitope

J Exp Med. 2021-08; 
Sarah A Greaves, Avinash Ravindran, Radleigh G Santos, Lan Chen, Michael T Falta, Yang Wang, Angela M Mitchell, Shaikh M Atif, Douglas G Mack, Alex N Tinega, Lisa A Maier, Shaodong Dai, Clemencia Pinilla, Johan Grunewald, Andrew P Fontenot
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Peptide Library Services … Individual peptides were synthesized using GenScript peptide library services. All peptides were dissolved in DMSO at high concentration … nidulans NDPD in serum, 96-well ELISA plates were coated with 10 μg/ml recombinant NDPD (GenScript) and incubated overnight at 4C. … Get A Quote

摘要

Löfgren's syndrome (LS) is an acute form of sarcoidosis characterized by a genetic association with HLA-DRB1*03 (HLA-DR3) and an accumulation of CD4+ T cells of unknown specificity in the bronchoalveolar lavage (BAL). Here, we screened related LS-specific TCRs for antigen specificity and identified a peptide derived from NAD-dependent histone deacetylase hst4 (NDPD) of Aspergillus nidulans that stimulated these CD4+ T cells in an HLA-DR3-restricted manner. Using ELISPOT analysis, a greater number of IFN-γ- and IL-2-secreting T cells in the BAL of DR3+ LS subjects compared with DR3+ control subjects was observed in response to the NDPD peptide. Finally, increased IgG antibody responses to A. nidulans NDPD were... More

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