Products/Services Used | Details | Operation |
---|---|---|
Peptide Library Services> | … Individual peptides were synthesized using GenScript peptide library services. All peptides were dissolved in DMSO at high concentration … nidulans NDPD in serum, 96-well ELISA plates were coated with 10 μg/ml recombinant NDPD (GenScript) and incubated overnight at 4C. … | Get A Quote |
Löfgren's syndrome (LS) is an acute form of sarcoidosis characterized by a genetic association with HLA-DRB1*03 (HLA-DR3) and an accumulation of CD4+ T cells of unknown specificity in the bronchoalveolar lavage (BAL). Here, we screened related LS-specific TCRs for antigen specificity and identified a peptide derived from NAD-dependent histone deacetylase hst4 (NDPD) of Aspergillus nidulans that stimulated these CD4+ T cells in an HLA-DR3-restricted manner. Using ELISPOT analysis, a greater number of IFN-γ- and IL-2-secreting T cells in the BAL of DR3+ LS subjects compared with DR3+ control subjects was observed in response to the NDPD peptide. Finally, increased IgG antibody responses to A. nidulans NDPD were... More