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Porcine Epidemic Diarrhea Virus Inhibits HDAC1 Expression To Facilitate Its Replication via Binding of Its Nucleocapsid Protein to Host Transcription Factor Sp1

J Virol. 2021-08; 
Jidong Xu, Junyong Mao, Xiao Han, Fushan Shi, Qin Gao, Tingjun Wang, Zhenning Zhang, Ying Shan, Weihuan Fang, Xiaoliang Li
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PCR Cloning and Subcloning … using the ClonExpress II One Step Cloning Kit (Vazyme, 377 Nanjing, China) and confirmed by DNA sequencing. The recombinant plasmid … Both proteins were expressed in soluble fractions and purified by GST or nickel resin 396 chromatography (Genscript, Nanjing, China) … Get A Quote

摘要

Porcine epidemic diarrhea virus (PEDV) is an enteric coronavirus causing acute intestinal infection in pigs, with high mortality often seen in neonatal pigs. The newborns rely on innate immune responses against invading pathogens because of lacking adaptive immunity. However, how PEDV disables the innate immunity of newborns toward severe infection remains unknown. We found that PEDV infection led to reduced expression of histone deacetylases (HDACs), especially HDAC1, in porcine IPEC-J2 cells. HDACs are considered important regulators of innate immunity. We hypothesized that PEDV interacts with certain host factors to regulate HDAC1 expression in favor of its replication. We show that HDAC1 acted as a negative... More

关键词

Sp1, histone deacetylase 1, nucleocapsid protein, porcine epidemic diarrhea virus, virus replication