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Tracing Clostridium perfringens strains from beef processing of slaughter house by pulsed-field gel electrophoresis, and the distribution and toxinotype of isolates in Shaanxi province, China

Food Microbiol. 2021-08; 
Yanfen Jiang, Yinghui Ma, Qianqian Liu, Tianmei Li, Yiming Li, Kangkang Guo, Yanming Zhang
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ORF cDNA Clones/MolecularCloud … Primers pairs modified and synthesized by GenScript (Nanjing, China) were used as shown in Table 1. The multiplex PCR was performed in a S1000™ thermoCycler (Bio-Rad, USA) described previous (Dong et al., 2013). Each single PCR assay had a final volume of 25 μL, 10 … Get A Quote

摘要

The purpose of this study was to investigate the distribution and specify the transmission and cross-contamination of Clostridium perfringens (C. perfringens) in the beef slaughtering and butchering process. The prevalence of 21.2% (150/708) yielded 208 isolates of C. perfringens, including 80.8% type A and 19.2% type D, 0.4% (3/708) samples carried both type A and D strains, and 72.5% type D isolates carried both cpe and atyp.cpb2 genes. C. perfringens were identified through the whole slaughtering process but no type F (cpe and cpa isolates) was found. 69 isolates were further analyzed and classified into 28 PFGE genotypes and clade I contained 94.2% isolates and 24 PFGE genotypes, which showed the genetic ... More

关键词

Beef slaughtering process, Clostridium perfringens, Contamination, PFGE, Toxinotype