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Downregulation of miR-1-3p expression inhibits the hypertrophy and mineralization of chondrocytes in DDH

J Orthop Surg Res. 2021-08; 
Rui Ding, Xijuan Liu, Jian Zhang, Jinghong Yuan, Sikuan Zheng, Xigao Cheng, Jingyu Jia
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Mutant Libraries … MiR-1-3p mimic (WT), miR-1-3p mutant (MUT), and negative control (NC) were cotransfected into human primary chondrocytes together with the Sox9 3′UTR luciferase reporter plasmid (GenScript) and Renilla reporter control. After 48 h, the cells were harvested using a Dual-… Get A Quote

摘要

background: Developmental dysplasia of the hip (DDH) is a highly prevalent hip disease among children. However, its pathogenesis remains unclear. MicroRNAs (miRNA) are important regulators of cartilage development. In a previous study, high-throughput miRNA sequencing of tissue samples from an animal model of DDH showed a low level of miR-1-3p in the cartilage of the acetabular roof (ARC), but its role in DDH pathogenesis was not addressed. Therefore, our aim here was to investigate the effects of miR-1-3p in the ARC. methods: The diagnosis of acetabular dysplasia was confirmed with X-ray examination, while imaging and HE staining were conducted to further evaluate the ARC thickness in each animal model. FISH w... More

关键词

Developmental dysplasia of the hip, Endochondral ossification, MicroRNA, SOX9, miR-1-3p