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MiR-199b-5p Promotes Gastric Cancer Progression by Regulating HHIP Expression

Front Oncol. 2021-08; 
Songda Chen, Huijie Wu, Lingyu Zhu, Mengjie Jiang, Shuli Wei, Jinhua Luo, Aiqun Liu
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Mutant Libraries … The 3 ‘- UTR sequence of HHIP containing mutants (MUT) or wild type (WT) miR - 199b - 5p binding sites was constructed by Genscript (Nanjing, China), and cloned into PGL - 3 luciferase reporting vector. After incubation in 24-well plates for 24 hours, pGL3 - WT - HHIP or … Get A Quote

摘要

unassigned: Gastric cancer (GC) is one of the most common malignant tumors. More and more evidences support the role of microRNAs (miRNAs) in tumor progression. However, the role of miRNAs in human GC remains largely unknown. unassigned: Based on the published gastric cancer expression profile data, combined with bioinformatics analysis, potential miRNAs in the process of GC were screened. The expression of miR-199b-5p in GC cells and patients' plasma was detected by RT-PCR. The effects of miR-199b-5p on GC were detected by EdU proliferation assay, colony formation assay, Transwell assay and wound healing assay. Western blot was used to detect epithelial-mesenchymal transition (EMT) related proteins. The sub... More

关键词

GC, HHIP, bioinformatics, maker, miR-199b-5p