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Double-Stranded RNA-Degrading Enzymes Reduce the Efficiency of RNA Interference in

Insects. 2021-08; 
Jin-Zhi Chen, Ying-Xia Jiang, Miao-Wen Li, Jian-Wen Li, Ben-Hu Zha, Guang Yang
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Monoclonal Antibody Services … incubated with His monoclonal antibody (GenScript, Nanjing, China), separately. The EstinTM L1 staining kit (GenScript, Nanjing, China) … After blocking with 5% w/v BSA (Solarbio, Beijing, China), each protein was incubated with His monoclonal antibody (GenScript, Nanjing, … Get A Quote

摘要

DsRNA-degrading enzymes (dsRNases) have been recognized as important factors in reducing RNA interference (RNAi) efficiency in different insect species. However, dsRNases in are still unknown. We identified the full-length cDNAs of , , , and . Gene expression profile showed that was mainly expressed in the hemolymph; and that and were mainly expressed in the intestinal tract. The expression of ( of ) in larvae injected with the mixture of dsPxCht (dsRNA of ) and dsPxdsRNase1 (dsRNA of ), dsPxdsRNase2 (dsRNA of ), or dsPxdsRNase3 (dsRNA of ) was significantly higher than that in the larvae injected with the mixture of dsGFP (dsRNA of green fluorescent protein gene, ) and dsPxCht; the transcription level of... More

关键词

RNA degradation, RNA interference, diamondback moth, dsRNase