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Semi-quantitative, high throughput analysis of SARS-CoV-2 neutralizing antibodies: Measuring the level and duration of immune response antibodies post infection/vaccination

Vaccine. 2021-08; 
Sean C Taylor, Beth Hurst, Ian Martiszus, Marvin S Hausman, Samar Sarwat, Jeffrey M Schapiro, Sarah Rowell, Alexander Lituev
Products/Services Used Details Operation
Monoclonal Antibody Services … Ten replicates of a standard curve using the kit insert (KI) “Standard Protocol” were compared to nine replicates from an optimized ambient temperature “Room Temp” procedure (see section 2.5) using a well-characterized neutralizing monoclonal antibody (MAB) (GenScript #… Get A Quote

摘要

The question associated with efficacy and longevity of SARS-CoV-2 protection post-vaccination is paramount. The cPass surrogate virus neutralization test (sVNT) has gained popularity globally as a dual application assay for: 1. Accurate SARS-CoV-2 population surveillance (seroprevalence) analysis and 2. Revealing the presence of antibodies that block and effectively neutralize the interaction between the SARS-CoV-2 receptor binding domain and the host cell ACE2 receptor in recovered or vaccinated individuals. This study describes an approach for accurate quantification of neutralizing antibodies using the cPass sVNT with an automated workflow on the Tecan EVO and Dynex Agility platforms that is applicable to ot... More

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