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Inhibition of Border Disease Virus Replication With Lentivirus-Mediated shRNAs

Front Vet Sci. 2021-08; 
Mohammad Javad Hajihasani Arani, Azam Mokhtari, Behnaz Saffar, Leila Asadi Samani
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Molecular Biology Tools … The sequences of the primers were designed using the GenScript real-time RT-PCR Primer Design web tool. They were as follows: forward: 5′-ATTCGTGCCCACCAGGAATA-3′, reverse: 5′-CAAGTTAGCCGGGTCCTCTC-3′, Bos taurus GAPDH forward: 5′- … Get A Quote

摘要

Border disease is believed to be one of the most important diseases in the animal husbandry industry, which has not yet been eradicated in Iran. The development of approaches based on the application of interfering RNA (RNAi) for antiviral therapy has attracted a great deal of attention over the recent years. The present research was conducted to design, construct, and apply shRNA against the NS3 gene of BDV to evaluate the prevention of BDV proliferation in the cell culture system. For this purpose, the suitable oligonucleotide sequence of NS3 gene coding was selected utilizing BDV- X818 strain. Afterwards, using shRNA design software, shRNA molecules were designed and synthesized. These shRNAs were cloned int... More

关键词

BDV, NS3, RNAi, TCID50, lentiviral plasmid, real-time PCR