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Phosphorylation of CrkL S114 induced by common gamma chain cytokines and T-cell receptor signal transduction

Sci Rep. 2021-08; 
Armando Estrada, Alejandro C Rodriguez, Georgialina Rodriguez, Alice H Grant, Yoshira M Ayala-Marin, Amy J Arrieta, Robert A Kirken
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Mammalian Expression … Anti-CrkL pS114 polyclonal containing antiserum was generated by Genscript using the human CrkL peptide sequence (MGSVpSAPNLPTAEDC). Dot blots with pS114 antibody were conducted using these same non-phosphorylated and phosphorylated peptides. Prior to … Get A Quote

摘要

T-cell activation and cellular expansion by common gamma chain cytokines such as Interleukin-2 is necessary for adaptive immunity. However, when unregulated these same pathways promote pathologies ranging from autoimmune disorders to cancer. While the functional role of Interleukin-2 and downstream effector molecules is relatively clear, the repertoire of phosphoregulatory proteins downstream of this pathway is incomplete. To identify phosphoproteins downstream of common gamma chain receptor, YT cells were radiolabeled with [P]-orthophosphate and stimulated with Interleukin-2. Subsequently, tyrosine phosphorylated proteins were immunopurified and subjected to tandem mass spectrometry-leading to the identificati... More

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