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Protective Efficacy of Recombinant Influenza Hemagglutinin Ectodomain Fusions

Viruses. 2021-08; 
Nidhi Mittal, Nayanika Sengupta, Sameer Kumar Malladi, Poorvi Reddy, Madhuraj Bhat, Raju S Rajmani, Koen Sedeyn, Xavier Saelens, Somnath Dutta, Raghavan Varadarajan
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Mammalian Expression … Genes for H1, H3 ectodomains (mMH1_02TE and mMH3_02TE, respectively) were human codon-optimized and cloned under control of the cytomegalovirus (CMV) promoter by GenScript Inc. (Piscataway, NJ, USA). The HA ectofusion construct genes (mMH3H1F_02TE and … Get A Quote

摘要

In current seasonal influenza vaccines, neutralizing antibody titers directed against the hemagglutinin surface protein are the primary correlate of protection. These vaccines are, therefore, quantitated in terms of their hemagglutinin content. Adding other influenza surface proteins, such as neuraminidase and M2e, to current quadrivalent influenza vaccines would likely enhance vaccine efficacy. However, this would come with increased manufacturing complexity and cost. To address this issue, as a proof of principle, we have designed genetic fusions of hemagglutinin ectodomains from H3 and H1 influenza A subtypes. These recombinant H1-H3 hemagglutinin ectodomain fusions could be transiently expressed at high yie... More

关键词

hemagglutinin, immunogen, influenza virus, linker, mouse immunization, mouse-adapted, neutralization, trimerization