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A virulent and pathogenic infectious clone of Senecavirus A

J Gen Virol. 2021-08; 
Maureen H V Fernandes, Marcelo de Lima, Lok R Joshi, Diego G Diel
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GenParts™ DNA Fragments … polymerase promoter T7 and the 5′end of SVA SD15-26 between the restriction enzymes NheI and SfiI and a pBrick plasmid containing the NheI restriction site followed by a DNA fragment encoding the P1, P2 and P3 regions of SVA SD15-26 were synthesized by Genscript. … Get A Quote

摘要

Senecavirus A (SVA) is a picornavirus that circulates in swine populations worldwide causing vesicular disease (VD) in affected animals. Here we developed a reverse genetics system for SVA based on the well-characterized wild-type SVA strain SD15-26 (wt SVA SD15-26). The full-length cDNA genome of SVA was cloned into a plasmid under a T7 RNA polymerase promoter. Following transcription, the genomic viral RNA was transfected into BHK-21 cells and rescue of infectious virus (rSVA SD15-26) was shown by inoculation of highly susceptible H1299 cells. characterization of the rSVA SD15-26 showed similar replication properties and protein expression levels as the wt SVA SD15-26. A pathogenesis study was conducted in ... More

关键词

SVA, SVV, Seneca Valley virus, infectious clone, recombinant, reverse genetics, vesicular disease