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Hydrophobic Moiety of Capsaicinoids Haptens Enhancing Antibody Performance in Immunoassay: Evidence from Computational Chemistry and Molecular Recognition

J Agric Food Chem. 2021-08; 
Yuchen Bai, Hui Jiang, Yingjie Zhang, Leina Dou, Minggang Liu, Wenbo Yu, Kai Wen, Jianzhong Shen, Yuebin Ke, Xuezhi Yu, Zhanhui Wang
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GenParts™ DNA Fragments … Bacteria liquid were sent to Genscript (Nanjing, China) for sequencing after the correct single clones were incubated overnight at 37 C in LB liquid medium with shaking at 250 rpm. The sanger sequencing results of single-chain variable fragment antibody (scFv) 2F7 and 26A1 … Get A Quote

摘要

We previously found that the immune response to haptens is positively correlated with molecular hydrophobicity. The antibodies used in immunoassays for capsaicinoids (CPCs) in waste oil suffer from low affinity and loose recognition to structural analogues. To address this issue, four new haptens (hapten1-4), maximally exposing the hydrophobic alkane chain (noncommon moiety of CPCs), were designed and expected to produce antibodies with high affinity and accurate recognition to CPCs based upon our findings. The assumption was first evidenced by computational chemistry and animal immunization successively. Compared with four reported haptens (hapten5-8) that expose the hydrophilic vanillyl amide moiety (common s... More

关键词

capsaicinoids, computational chemistry, hapten design, hydrophobicity, molecular recognition