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Berberine ameliorates nonalcoholic fatty liver disease by decreasing the liver lipid content via reversing the abnormal expression of MTTP and LDLR

Exp Ther Med. 2021-08; 
Ping Chen, Yusheng Li, Li Xiao
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DNA Sequencing … Next, the diluted cDNA (2 µl) was subjected to qPCR using SYBR-Green I (Takara Bio, Inc.) with primers synthesized by Nanjing GenScript Co., Ltd. The mRNA expression levels were normalized to the housekeeping gene β-actin. The primer sequences were: MTTP, forward 5'-… Get A Quote

摘要

The global incidence of nonalcoholic fatty liver disease (NAFLD) is increasing. The present study explored the effect and mechanism of berberine (BBR) on NAFLD in rats. Thirty-five Sprague-Dawley rats were randomly divided into the control and NAFLD groups, which were fed a normal diet or high-fat diet, respectively, for 8 weeks. Hematoxylin and eosin staining was performed on liver tissues and establishment of the NAFLD model was confirmed by microscopy. NAFLD rats were subsequently randomly subdivided and treated with saline or BBR for 8 weeks. The liver wet weight of rats in each group was measured, the liver tissue structure was observed by microscopy, and alanine aminotransferase (ALT), aspartate aminotran... More

关键词

apolipoprotein B, berberine, low-density lipoprotein receptor, microsomal triglyceride transfer protein, nonalcoholic fatty liver disease