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mRNA-1273 protects against SARS-CoV-2 beta infection in nonhuman primates

Nat Immunol. 2021-08; 
Kizzmekia S Corbett, Anne P Werner, Sarah O' Connell, Matthew Gagne, Lilin Lai, Juan I Moliva, Barbara Flynn, Angela Choi, Matthew Koch, Kathryn E Foulds, Shayne F Andrew, Dillon R Flebbe, Evan Lamb, Saule T Nurmukhambetova, Samantha J Provost, Kevin W Bock, Mahnaz Minai, Bianca M Nagata, Alex Van Ry, Zackery Flinchbaugh, Timothy S Johnston, Elham Bayat Mokhtari, Prakriti Mudvari, Amy R Henry, Farida Laboune, Becky Chang, Maciel Porto, Jaclyn Wear, Gabriela S Alvarado, Seyhan Boyoglu-Barnum, John-Paul M Todd, Bridget Bart, Anthony Cook, Alan Dodson, Laurent Pessaint, Katelyn Steingrebe, Sayda Elbashir, Manjari Sriparna, Andrew Pekosz, Hanne Andersen, Kai Wu, Darin K Edwards, Swagata Kar, Mark G Lewis, Eli Boritz, Ian N Moore, Andrea Carfi, Mehul S Suthar, Adrian McDermott, Mario Roederer, Martha C Nason, Nancy J Sullivan, Daniel C Douek, Barney S Graham, Robert A Seder
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DNA Sequencing … Total RNA was extracted from nasal specimens and BAL fluid using RNAzol BD Column Kits and eluted in 65 μl water. Subgenomic SARS-… For the standard curve, the E or N subgenomic mRNA sequence was inserted into a pcDNA3.1 vector (GenScript) and transcribed using … Get A Quote

摘要

B.1.351 is the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variant most resistant to antibody neutralization. We demonstrate how the dose and number of immunizations influence protection. Nonhuman primates received two doses of 30 or 100 µg of Moderna's mRNA-1273 vaccine, a single immunization of 30 µg, or no vaccine. Two doses of 100 µg of mRNA-1273 induced 50% inhibitory reciprocal serum dilution neutralizing antibody titers against live SARS-CoV-2 p.Asp614Gly and B.1.351 of 3,300 and 240, respectively. Higher neutralizing responses against B.1.617.2 were also observed after two doses compared to a single dose. After challenge with B.1.351, there was ~4- to 5-log reduction of viral s... More

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