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DNA Sequencing> | … Total RNA was extracted from nasal specimens and BAL fluid using RNAzol BD Column Kits and eluted in 65 μl water. Subgenomic SARS-… For the standard curve, the E or N subgenomic mRNA sequence was inserted into a pcDNA3.1 vector (GenScript) and transcribed using … | Get A Quote |
B.1.351 is the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variant most resistant to antibody neutralization. We demonstrate how the dose and number of immunizations influence protection. Nonhuman primates received two doses of 30 or 100 µg of Moderna's mRNA-1273 vaccine, a single immunization of 30 µg, or no vaccine. Two doses of 100 µg of mRNA-1273 induced 50% inhibitory reciprocal serum dilution neutralizing antibody titers against live SARS-CoV-2 p.Asp614Gly and B.1.351 of 3,300 and 240, respectively. Higher neutralizing responses against B.1.617.2 were also observed after two doses compared to a single dose. After challenge with B.1.351, there was ~4- to 5-log reduction of viral s... More