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DNA Sequencing> | … control RNAs were chemosynthetic and purified with HPLC (GenScript, China) according to the reported sequence. Lentiviruses inserted … were constructed and purified to 1 × 10 9 CFU by HanBio, China. The operation of lentivirus transfection of ESCC cells and the operation of … | Get A Quote |
Esophageal squamous cell carcinoma (ESCC) is one of the most common gastrointestinal tumors, accounting for almost half a million deaths per year. Cancer-associated fibroblasts (CAFs) are the major constituent of the tumor microenvironment (TME) and dramatically impact ESCC progression. Recent evidence suggests that exosomes derived from CAFs are able to transmit regulating signals and promote ESCC development. In this study, we compared different the component ratios of miRNAs in exosomes secreted by CAFs in tumors and with those from normal fibroblasts (NFs) in precancerous tissues. The mRNA level of hsa-miR-3656 was significantly upregulated in the former exosomes. Subsequently, by comparing tumor cell devel... More