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CRISPR/Cas9-engineered inducible gametocyte producer lines as a valuable tool for Plasmodium falciparum malaria transmission research

Nat Commun. 2021-08; 
Sylwia D Boltryk, Armin Passecker, Arne Alder, Eilidh Carrington, Marga van de Vegte-Bolmer, Geert-Jan van Gemert, Alex van der Starre, Hans-Peter Beck, Robert W Sauerwein, Taco W A Kooij, Nicolas M B Brancucci, Nicholas I Proellochs, Tim-Wolf Gilberger, Till S Voss
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DNA Sequencing … , a more pronounced gene expression signature emerges in the sexual ring stage progeny, where several dozen genes are specifically … falciparum codon-adjusted synthetic sequence (Genscript) (Supplementary Fig. ) using primers 14 F and 14 R), (3) the P. falciparum histidine-… Get A Quote

摘要

The malaria parasite Plasmodium falciparum replicates inside erythrocytes in the blood of infected humans. During each replication cycle, a small proportion of parasites commits to sexual development and differentiates into gametocytes, which are essential for parasite transmission via the mosquito vector. Detailed molecular investigation of gametocyte biology and transmission has been hampered by difficulties in generating large numbers of these highly specialised cells. Here, we engineer P. falciparum NF54 inducible gametocyte producer (iGP) lines for the routine mass production of synchronous gametocytes via conditional overexpression of the sexual commitment factor GDV1. NF54/iGP lines consistently achieve ... More

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