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Engineering entropy-driven based multiple signal amplification strategy for visualized assay of miRNA by naked eye

Talanta. 2021-08; 
Sha Zhu, Yi Qi Yang, Yuedi Ding, Ninghan Feng, Menglu Li, Yongmei Yin
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DNA Sequencing … The oligonucleotides used in this study were synthesized and purified by Genscript Co., Ltd. (Nanjing, China) with the sequences shown in Table S1. Nicking endonuclease (Nb.BbvCI) was purchased from New England Biolabs Ltd. … Get A Quote

摘要

MicroRNAs (miRNAs) are currently recognized as novel biomarkers for cancer early diagnosis, therapy selection, and progression monitoring. Herein, we developed an ultrasensitive and label-free homogeneous colorimetric strategy for miRNA detection based on engineering entropy-driven amplification (EDA) coupled with nicking enzyme-assisted AuNP aggregation. In our design, the target miRNA could specifically trigger the EDA recycling process. One of the EDA products could open the hairpin probe and form a dual strand containing a nicking endonuclease (Nb.BbvCl) cleavage region. After adding nicking endonuclease in the sensing solution, the product DNA fragments could act as two linkers, inducing the aggregation of... More

关键词

Colorimetric biosensor, Entropy-driven, Gold nanoparticle, MicroRNA detection, Multiple signal amplification, Nicking enzyme