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Custom Vector Construction> | … The corresponding gene was synthesized by Genscript, USA and cloned into pET28a(+) bacterial vector by using NdeI and BamHI restriction cleavage sites at 5’ and 3’ ends respectively. N-terminal canonical SARS-CoV-2 Mpro autocleavage cut site is indicated by green and … | Get A Quote |
The COVID-19 pandemic has resulted in 198 million reported infections and more than 4 million deaths as of July 2021 (covid19.who.int). Research to identify effective therapies for COVID-19 includes: (1) designing a vaccine as future protection; (2) de novo drug discovery; and (3) identifying existing drugs to repurpose them as effective and immediate treatments. To assist in drug repurposing and design, we determine two apo structures of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) main protease at ambient temperature by serial femtosecond X-ray crystallography. We employ detailed molecular simulations of selected known main protease inhibitors with the structures and compare binding modes and ... More