至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

An epigenetic basis of inbreeding depression in maize

Sci Adv. 2021-08; 
Tongwen Han, Fang Wang, Qingxin Song, Wenxue Ye, Tieshan Liu, Liming Wang, Z Jeffrey Chen
Products/Services Used Details Operation
Custom Vector Construction … The IR (table S6) designed according to the promoter sequence of Zm00001d015449 was synthesized by GenScript company and cloned into the cucumber mosaic virus (CMV) VIGS (pCMV201) vector to construct the VIGS-IR plasmid. Plasmids pCMV101, pCMV301, VIGS-IR, … Get A Quote

摘要

Inbreeding depression is widespread across plant and animal kingdoms and may arise from the exposure of deleterious alleles and/or loss of overdominant alleles resulting from increased homozygosity, but these genetic models cannot fully explain the phenomenon. Here, we report epigenetic links to inbreeding depression in maize. Teosinte branched1/cycloidea/proliferating cell factor (TCP) transcription factors control plant development. During successive inbreeding among inbred lines, thousands of genomic regions across TCP-binding sites (TBS) are hypermethylated through the H3K9me2-mediated pathway. These hypermethylated regions are accompanied by decreased chromatin accessibility, increased levels of the repres... More

关键词