Products/Services Used | Details | Operation |
---|---|---|
Custom Vector Construction> | … The IR (table S6) designed according to the promoter sequence of Zm00001d015449 was synthesized by GenScript company and cloned into the cucumber mosaic virus (CMV) VIGS (pCMV201) vector to construct the VIGS-IR plasmid. Plasmids pCMV101, pCMV301, VIGS-IR, … | Get A Quote |
Inbreeding depression is widespread across plant and animal kingdoms and may arise from the exposure of deleterious alleles and/or loss of overdominant alleles resulting from increased homozygosity, but these genetic models cannot fully explain the phenomenon. Here, we report epigenetic links to inbreeding depression in maize. Teosinte branched1/cycloidea/proliferating cell factor (TCP) transcription factors control plant development. During successive inbreeding among inbred lines, thousands of genomic regions across TCP-binding sites (TBS) are hypermethylated through the H3K9me2-mediated pathway. These hypermethylated regions are accompanied by decreased chromatin accessibility, increased levels of the repres... More