Products/Services Used | Details | Operation |
---|---|---|
Custom Vector Construction> | … The reaction product was dialyzed (dialysis membrane Spectra/Por®, cut-off 15,000, Spectrum Laboratories Inc., Rancho Dominguez, CA, USA… S1), was purchased from GenScript (Netherlands) and cloned at NdeI-BamHI restriction sites in a pET29-a(+) expression vector. Such … | Get A Quote |
Advanced biomaterials provide an interesting and versatile platform to implement new and more effective strategies to fight bacterial infections. Chitosan is one of these biopolymers and possesses relevant features for biomedical applications. Here we synthesized nanoparticles of chitosan derivatized with diethylaminoethyl groups (ChiDENPs) to emulate the choline residues in the pneumococcal cell wall and act as ligands for choline-binding proteins (CBPs). Firstly, we assessed the ability of diethylaminoethyl (DEAE) to sequester the CBPs present in the bacterial surface, thus promoting chain formation. Secondly, the CBP-binding ability of ChiDENPs was purposed to encapsulate a bio-active molecule, the antimicro... More