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Merlin functions as a critical regulator in Staphylococcus aureus-induced osteomyelitis

J Cell Physiol. 2021-08; 
Zubin Zhou, Yuanliang Chen, Hong Sung Min, Yongbai Wan, Haojie Shan, Yiwei Lin, Wenyang Xia, Fuli Yin, Chaolai Jiang, Xiaowei Yu
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Custom DNA/RNA Oligos … The primers were synthesized by GenScript. The sequence of primers is listed as follows: Il6 sense: 5ʹ-CAC AGA GGA TAC CAC TCC CAA CA-3ʹ and antisense: 5ʹ-TCC ACG ATT TCC CAG AGA ACA-3ʹ; Tnf sense: 5ʹ-CAT CTT CTC AAA ATT CGA GTG ACA A-3ʹ and … Get A Quote

摘要

Merlin is known as a tumor suppressor, while its role in osteomyelitis remains unclear. This study aimed to investigate the role of Merlin in Staphylococcus aureus-induced osteomyelitis and its underlying mechanisms. S. aureus-induced osteomyelitis mouse model was established in Merlin Lyz2 and Merlin Lyz2 mice. Bone marrow-derived macrophages (BMDMs) were isolated and stimulated by lipopolysaccharide (LPS). Bioassays, including quantitative reverse transcription polymerase chain reaction (qRT-PCR), Western blot analysis, and enzyme-linked immunosorbent assays, were conducted to determine the levels of target genes or proteins. Immunoprecipitation was applied to determine the interactions between proteins. DCAF... More

关键词

DCAF1, Merlin, Staphylococcus aureus, inflammatory response, osteomyelitis