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Interferon-beta, interferon-gamma and their fusion interferon of Siberian tigers (Panthera tigris altaica) in China are involved in positive-feedback regulation of interferon production

Dev Comp Immunol. 2021-07; 
Mengyao Mu, Hongjing Zhao, Yu Wang, Menghao Guo, Xiaopan Nie, Yachen Liu, Mingwei Xing
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Recombinant Antibody Expression … The recombinant proteins were purified with a High Affinity Ni-Charged Resin (GenScript, Nanjing, China) as previously described. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis was used to determine the expression form of recombinant … Get A Quote

摘要

As a group of cytokines, interferons are the first line of defense in the antiviral immunity. In this study, Siberian tiger IFN-β (PtIFN-β) and IFN-γ (PtIFN-γ) were successfully amplified, and the two were fused (PtIFN-γ) by overlap extension polymerase chain reaction (SOE-PCR). Bioinformatics analysis disclosed that PtIFN-β and PtIFN-γ have species-specificity and conservation in the course of evolution. After being expressed in prokaryotes, the antiviral activities and physicochemical properties of PtIFN-β, PtIFN-γ and PtIFNβ-γ were analyzed. In Feline kidney cells (F81), PtIFNβ-γ showed more active antiviral activity than PtIFN-β and PtIFN-γ, which has more stable physicochemical properties (a... More

关键词

Antiviral activity, Interferon β, Interferon γ, JAK-STAT pathway, Overlap extension PCR, Panthera tigris altaica