Products/Services Used | Details | Operation |
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Recombinant Antibody Expression | … S protein to recombinant ACE2 (Genscript Biotech, USA). Inhibition percentage ≥25% in a sample was considered as positive for ACE2-blocking antibodies. This assay was found to be 100% specific for measuring ACE2-blocking antibodies in the Sri Lankan population 23 . … | Get A Quote |
Several COVID-19 vaccines have received emergency approval. Here we assess the immunogenicity of a single dose of the AZD1222 vaccine, at one month, in a cohort of health care workers (HCWs) (629 naïve and 26 previously infected). 93.4% of naïve HCWs seroconverted, irrespective of age and gender. Haemagglutination test for antibodies to the receptor binding domain (RBD), surrogate neutralization assay (sVNT) and ex vivo IFNγ ELISpot assays were carried out in a sub-cohort. ACE2 blocking antibodies (measured by sVNT) were detected in 67/69 (97.1%) of naïve HCWs. Antibody levels to the RBD of the wild-type virus were higher than to RBD of B.1.1.7, and titres to B.1.351 were very low. Ex vivo T cell responses ... More