Products/Services Used | Details | Operation |
---|---|---|
Recombinant Antibody Expression> | … within the purified recombinant protein samples was removed using the ToxinEraser TM Endotoxin Removal Kit (GenScript, China), and then residual endotoxin (< 0.3 EU/mL) was tested using the ToxinSensor TM Chromogenic LAL Endotoxin Assay Kit (GenScript, China). … | Get A Quote |
As a proinflammatory cytokine of the interleukin-1 (IL-1) family, IL-18 plays important roles in host protection against bacterial, viral, and fungal infection. We cloned the open reading frame of snakehead (Channa argus) IL-18 (shIL-18) and found that it contained 609 base pairs and encoded 202 amino acid residues. The shIL-18 included a conserved IL-1-like family signature and two potential IL-1β-converting enzyme cutting sites; one was conserved in all analyzed IL-18s, but the other was unique to shIL-18. Unlike other IL-18s, shIL-18 also contained a predicted signal peptide. In this study, shIL-18 was constitutively expressed in all tested tissues, and its expression was induced by Aeromonas schubertii and... More