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Comparison of alternative integration sites in the chromosome and the native plasmids of the cyanobacterium Synechocystis sp PCC 6803 in respect to expression efficiency and copy number

Microb Cell Fact. 2021-07; 
Csaba Nagy, Kati Thiel, Edita Mulaku, Henna Mustila, Paula Tamagnini, Eva-Mari Aro, Catarina C Pacheco, Pauli Kallio
Products/Services Used Details Operation
PCR Cloning and Subcloning … coli vector pUC57 (GenScript). The plasmid contains separate multiple cloning sites for the incorporation of the alternative upstream and downstream homologous sequences corresponding to the different sites of integration, which were PCR-amplified from the host genome … Get A Quote

摘要

background: Synechocystis sp. PCC 6803 provides a well-established reference point to cyanobacterial metabolic engineering as part of basic photosynthesis research, as well as in the development of next-generation biotechnological production systems. This study focused on expanding the current knowledge on genomic integration of expression constructs in Synechocystis, targeting a range of novel sites in the chromosome and in the native plasmids, together with established loci used in literature. The key objective was to obtain quantitative information on site-specific expression in reference to replicon copy numbers, which has been speculated but never compared side by side in this host. results: An optimized s... More

关键词

Genomic integration, Native plasmids, Replicon copy number, Synechocystis sp. PCC 6803, sYFP2