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PCR Cloning and Subcloning> | … Genes used in this study were codon-optimized for mammalian expression (IDT codon optimization tool), synthesized by GenScript, and cloned into pcDNA 3.1 (pcDx) expression vectors by FX-cloning (Geertsma and Dutzler, 2011). rXKR9 (molecular weight (MW) 43.6 kDa) … | Get A Quote |
The exposure of the negatively charged lipid phosphatidylserine on the cell surface, catalyzed by lipid scramblases, is an important signal for the clearance of apoptotic cells by macrophages. The protein XKR9 is a member of a conserved family that has been associated with apoptotic lipid scrambling. Here, we describe structures of full-length and caspase-treated XKR9 from in complex with a synthetic nanobody determined by cryo-electron microscopy. The 43 kDa monomeric membrane protein can be divided into two structurally related repeats, each containing four membrane-spanning segments and a helix that is partly inserted into the lipid bilayer. In the full-length protein, the C-terminus interacts with a hydrop... More