Products/Services Used | Details | Operation |
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PCR Cloning and Subcloning> | … All sequences were codon optimized for expression in human cells prior to synthesis and subcloning into a proprietary modified phCMV plasmid at Genscript (Piscataway, NJ). eVLPs were produced by transient polyethylenimine transfection in proprietary HEK-293SF-3F6 GMP … | Get A Quote |
We evaluated enveloped virus-like particles (eVLPs) expressing various forms of the Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) spike protein and several adjuvants in an effort to identify a highly potent Coronavirus disease 2019 (COVID-19) vaccine candidate. eVLPs expressing a modified prefusion form of SARS-CoV-2 spike protein were selected as they induced high antibody binding titers and neutralizing activity after a single injection in mice. Formulation of SARS-CoV-2 S eVLPs with aluminum phosphate resulted in balanced induction of IgG2 and IgG1 isotypes and antibody binding and neutralization titers were undiminished for more than 3 months after a single immunization. A single dose of thi... More