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HBx represses WDR77 to enhance HBV replication by DDB1-mediated WDR77 degradation in the liver

Theranostics. 2021-07; 
Hongfeng Yuan, Lina Zhao, Ying Yuan, Haolin Yun, Wei Zheng, Yu Geng, Guang Yang, Yufei Wang, Man Zhao, Xiaodong Zhang
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Nucleic Acid Purification & Analysis … All the plasmids used in this study were constructed by Genscript Biotechnology Co., Ltd (Nanjing, China) unless specifically stated. The … First-strand cDNA was synthesized using the Hifair Ⅲ 1st strand cDNA synthesis supermix kit (Yeasen Biotech, Shanghai, China), in which … Get A Quote

摘要

Hepatitis B x protein (HBx) is required to initiate and maintain the replication of hepatitis B virus (HBV). Protein arginine methyltransferases 5 (PRMT5) negatively regulates HBV transcription. WD repeat domain 77 protein (WDR77) greatly enhances the methyltransferase activity of PRMT5. However, the role of WDR77 in the modulation of cccDNA transcription and HBV replication is poorly understood. In this study, we investigated the mechanism by which HBx modulated HBV replication involving WDR77 in the liver. A human liver-chimeric mouse model was established. Immunohistochemistry (IHC) staining, Western blot analysis, Southern blot analysis, Northern blot analysis, immunofluorescence assays, ELISA, RT-qPCR, Co... More

关键词

DDB1, H4R3me2s, HBx, PRMT5, WDR77