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MicroRNA-138 Increases Chemo-Sensitivity of Glioblastoma through Downregulation of Survivin

Biomedicines. 2021-07; 
Ji-Young Yoo, Margaret Yeh, Yin-Ying Wang, Christina Oh, Zhong-Ming Zhao, Balveen Kaur, Tae-Jin Lee
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Mutagenesis Services … within the seed sequences to cytosines using site-directed mutagenesis kit (GenScript, New Jersey, NJ, USA). The plasmid DNAs were amplified from E.coli were, then purified by DNA Maxi kit (Qiagen, Germantown, MD, USA). GBM cells plated in 12 well plates one day before … Get A Quote

摘要

Glioblastoma (GBM) is one of the most deadly cancers and poorly responses to chemotherapies, such as temozolomide (TMZ). Dysregulation of intrinsic signaling pathways in cancer cells are often resulted by dysregulated tumor suppressive microRNAs (miRNAs). Previously, we found miR-138 as one of tumor suppressive miRNAs that were significantly down-regulated in GBM. In this study, we demonstrated that ectopic over-expression of miR-138 sensitizes GBM cells to the treatment of TMZ and increased apoptotic cell death. Mechanistically, miR-138 directly repressed the expression of Survivin, an anti-apoptotic protein, to enhance caspase-induced apoptosis upon TMZ treatment. Using an intracranial GBM xenograft mice mode... More

关键词

BIRC5, glioblastoma (GBM), microRNA-138 (miR-138), survivin, temozolomide (TMZ)