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Immunological studies on chicken interferon-kappa using an antigen-capture ELISA developed using new mouse monoclonal antibodies

Dev Comp Immunol. 2021-07; 
Youngsub Lee, Mingmin Lu, Hyun S Lillehoj
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Large-Scale Antibody Services … of immunization with chIFN-κ in mice and fusion for hybridoma generation were performed by Genscript Inc. (Piscataway, NJ, USA). … Louis, MO, USA) and the OD was measured at 450 nm using a microplate reader (BioTek, VT, USA). All plate washing steps were performed with … Get A Quote

摘要

Interferon (IFN)-κ is a type I IFN that plays a central role in anti-viral defense and host immune response. The functions of type I IFNs have not been clearly defined in chickens compared to those of their mammalian counterparts. In this study, we developed an antigen-capture ELISA using newly developed mouse monoclonal antibodies (mAbs) which are specific for chicken IFN-κ (chIFN-κ) and showed that this ELISA can measure native chIFN-κ production during the activation of macrophages by polyinosinic:polycytidylic acid (poly I:C). The recombinant chicken IFN-κ expressed in Escherichia coli was used to immunize mice. Five mAbs that specifically recognized chIFN-κ were selected and characterized based on th... More

关键词

Capture ELISA, IFN-κ, Monoclonal antibody, Type Ι IFNs, mRNA