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Unconventional Spidroin Assemblies in Aqueous Dope for Spinning into Tough Synthetic Fibers

ACS Biomater Sci Eng. 2021-07; 
Chun-Fei Hu, Zhi-Gang Qian, Qingfa Peng, Yaopeng Zhang, Xiao-Xia Xia
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GenParts™ DNA Fragments … ACF19411.1) was synthesized and delivered on plasmid pUC57-MaSp1-Nterm from GenScript (Nanjing, China). The DNA fragment encoding mature NTD (without the secretion leader peptide) was then PCR-amplified from pUC57-MaSp1-Nterm with primers FmNNhe (5′-… Get A Quote

摘要

Spider dragline silk is a remarkable fiber made by spiders from an aqueous solution of spidroins, and this feat is largely attributed to the tripartite domain architecture of the silk proteins leading to the hierarchical assembly at the nano- and microscales. Although individual amino- and carboxy-terminal domains have been proposed to relate to silk protein assembly, their tentative synergizing roles in recombinant spidroin storage and spinning into synthetic fibers remain elusive. Here, we show biosynthesis and self-assembly of a mimic spidroin composed of amino- and carboxy-terminal domains bracketing 16 consensus repeats of the core region from spider . The presence of both termini was found essential for s... More

关键词

biosynthesis, microfluidic spinning, spider silk, terminal domains, tough fibers