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Characterization of CRISPR array in Salmonella enterica from asymptomatic people and patients

Int J Food Microbiol. 2021-07; 
Kai Zhang, Yue Zhang, Zhenyu Wang, Yang Li, Haiyan Xu, Xinan Jiao, Qiuchun Li
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DNA Sequencing … Then, 5 μL of the PCR product was subjected to electrophoresis at 1% agarose gel for identification, and the remaining PCR products were subsequently sent to Genscript Biotech Corporation for sequencing using the ABI3730 apparatus (Applied Biosystems). … Get A Quote

摘要

Salmonella enterica is a major foodborne pathogen causing symptomatic diseases or asymptomatic infections in humans. To reveal the genetic difference of Salmonella strains from patients to that from asymptomatic people, we used CRISPR typing to analyze the phylogenetic relationship of 180 clinical strains during 2017-2018 in Jiangsu, China. The CRISPR typing divided these isolates into 76 CRISPR types with a discriminatory power of 97.6%. S. Typhimurium and its monophasic variants of 6 CRISPR types are the significant serotypes causing both human diseases and asymptomatic infection, while S. Enteritidis mainly resulted in diseases and shared one CRISPR type. The spacer HadB20 displayed as a new molecular marker... More

关键词

CRISPR typing, Evolution, Salmonella enterica, Spacer