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m(6)A Modification of lncRNA NEAT1 Regulates Chronic Myelocytic Leukemia Progression miR-766-5p/CDKN1A Axis

Front Oncol. 2021-07; 
Fang-Yi Yao, Cui Zhao, Fang-Min Zhong, Ting-Yu Qin, Fang Wen, Mei-Yong Li, Jing Liu, Bo Huang, Xiao-Zhong Wang
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DNA Sequencing … All primers were designed and synthesized by Nanjing Genscript Biotech Co., Ltd.; GAPDH and U6 were used for internal reference. Fold changes of the indicated genes were calculated using 2 -ΔΔCt method. The sequences of the primers used were as follows: NEAT1: F, 5′-… Get A Quote

摘要

unassigned: Chronic myeloid leukemia (CML) is an acquired hematopoietic stem malignant disease originating from the myeloid system. Long non-coding RNAs (lncRNAs) have been widely explored in cancer tumorigenesis. However, their roles in CML remain largely unclear. unassigned: The peripheral blood mononuclear cells (PBMCs) and CML cell lines (K562, KCL22, MEG01, BV173) were collected for research. Real-time quantitative polymerase chain reaction was used to determine the mRNA expression levels. Cell viability and apoptosis were analyzed by cell counting kit 8 and flow cytometry assays. The targeting relationships were predicted using Starbase and TargetScan and ulteriorly verified by RNA pull-down and lucifera... More

关键词

CDKN1A, METTL3, N6-methyladenosine (m6A) modification, NEAT1, chronic myeloid leukemia, miR-766-5p