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A 5'-tRNA halve, tiRNA-Gly promotes cell proliferation and migration via binding to RBM17 and inducing alternative splicing in papillary thyroid cancer

J Exp Clin Cancer Res. 2021-07; 
Litao Han, Hejing Lai, Yichen Yang, Jiaqian Hu, Zhe Li, Ben Ma, Weibo Xu, Wanlin Liu, Wenjun Wei, Duanshu Li, Yu Wang, Qiwei Zhai, Qinghai Ji, Tian Liao
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DNA Sequencing … Fold changes in expression were calculated using the 2- ΔΔ Ct method. … In brief, biotin-labeled tiRNA-Gly (sense) and antisense were synthesized by GenScript. The RNA bait sequences were heated to 99 C and placed on ice immediately to eliminate secondary structure. 50 μl … Get A Quote

摘要

background: tRNA-derived small noncoding RNAs (sncRNAs) are mainly categorized into tRNA halves (tiRNAs) and fragments (tRFs). Biological functions of tiRNAs in human solid tumor are attracting more and more attention, but researches concerning the mechanisms in tiRNAs-mediated tumorigenesis are rarely. The direct regulatory relationship between tiRNAs and splicing-related proteins remain elusive. methods: Papillary thyroid carcinoma (PTC) associated tRNA fragments were screened by tRNA fragments deep sequencing and validated by qRT-PCR and Northern Blot in PTC tissues. The biological function of tRNA fragments were assessed by cell counting kit, transwells and subcutaneous transplantation tumor of nude mice. F... More

关键词

Alternative splicing, MAP4K4, Papillary thyroid carcinoma, RBM17, tiRNA-Gly