| Products/Services Used | Details | Operation |
|---|---|---|
| Custom Vector Construction | … The reporter plasmids were designed by Genscript (Nanjing, China). Briefly, cells were transfected with firefly luciferase reporter plasmids, β‐galactosidase expression vectors, and the same amount of mimics, inhibitors, or scrambled negative control (NC) RNA for luciferase … | Get A Quote |
MicroRNAs (miRNAs) are involved in the progression of many cancers through largely unelucidated mechanisms. The results of our present study identified a gene cluster, miR-221/222, that is constitutively upregulated in serum exosome samples of patients with colorectal carcinoma (CRC) with liver metastasis (LM); this upregulation predicts a poor overall survival rate. Using an in vitro cell coculture model, we demonstrated that CRC exosomes harboring miR-221/222 activate liver hepatocyte growth factor (HGF) by suppressing SPINT1 expression. Importantly, miR-221/222 plays a key role in forming a favorable premetastatic niche (PMN) that leads to the aggressive nature of CRC, which was further shown through in vivo... More
