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Retrospective cell lineage reconstruction in humans by using short tandem repeats

Cell Rep Methods. 2021-07; 
Liming Tao, Ofir Raz, Zipora Marx, Manjusha S Ghosh, Sandra Huber, Julia Greindl-Junghans, Tamir Biezuner, Shiran Amir, Lilach Milo, Rivka Adar, Ron Levy, Amos Onn, Noa Chapal-Ilani, Veronika Berman, Asaf Ben Arie, Guy Rom, Barak Oron, Ruth Halaban, Zbigniew T Czyz, Melanie Werner-Klein, Christoph A Klein, Ehud Shapiro
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Custom DNA/RNA Oligos … OM6 or OM7 pools (lists of the oligonucleotides comprised in each of the duplex MIP designs is listed in the Data S1 were synthesized by Custom Array (GenScript), Inc. The pools were diluted to a final concentration of 1ng/μl and used as templates. PCR primers (OM4_Mly_F:5`… Get A Quote

摘要

Cell lineage analysis aims to uncover the developmental history of an organism back to its cell of origin. Recently, novel methods utilizing genome editing enabled important insights into the cell lineages of animals. In contrast, human cell lineage remains restricted to retrospective approaches, which still lack resolution and cost-efficient solutions. Here, we demonstrate a scalable platform based on short tandem repeats targeted by duplex molecular inversion probes. With this human cell lineage tracing method, we accurately reproduced a known lineage of DU145 cells and reconstructed lineages of healthy and metastatic single cells from a melanoma patient who matched the anatomical reference while adding furt... More

关键词

cell lineage reconstruction, molecular inversion probes, short tandem repeats, single-cell genomics