Products/Services Used | Details | Operation |
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Custom DNA/RNA Oligos> | … OM6 or OM7 pools (lists of the oligonucleotides comprised in each of the duplex MIP designs is listed in the Data S1 were synthesized by Custom Array (GenScript), Inc. The pools were diluted to a final concentration of 1ng/μl and used as templates. PCR primers (OM4_Mly_F:5`… | Get A Quote |
Cell lineage analysis aims to uncover the developmental history of an organism back to its cell of origin. Recently, novel methods utilizing genome editing enabled important insights into the cell lineages of animals. In contrast, human cell lineage remains restricted to retrospective approaches, which still lack resolution and cost-efficient solutions. Here, we demonstrate a scalable platform based on short tandem repeats targeted by duplex molecular inversion probes. With this human cell lineage tracing method, we accurately reproduced a known lineage of DU145 cells and reconstructed lineages of healthy and metastatic single cells from a melanoma patient who matched the anatomical reference while adding furt... More