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Essential gene acquisition destabilizes plasmid inheritance

PLoS Genet. 2021-07; 
Tanita Wein, Yiqing Wang, Myriam Barz, Fenna T Stücker, Katrin Hammerschmidt, Tal Dagan
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Bacterial Expression … The plasmid-derived GroE (WP_004201172.1, WP_004201176.1) was custom synthesized by Genscript and comprised the sequence of the strain E. coli 6409 (NZ_CP020056.1; Fig 2C). The sequence was introduced into pCON-S by PCR amplification (for primers see S6 … Get A Quote

摘要

Extra-chromosomal genetic elements are important drivers of evolutionary transformations and ecological adaptations in prokaryotes with their evolutionary success often depending on their 'utility' to the host. Examples are plasmids encoding antibiotic resistance genes, which are known to proliferate in the presence of antibiotics. Plasmids carrying an essential host function are recognized as permanent residents in their host. Essential plasmids have been reported in several taxa where they often encode essential metabolic functions; nonetheless, their evolution remains poorly understood. Here we show that essential genes are rarely encoded on plasmids; evolving essential plasmids in Escherichia coli we furthe... More

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