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Tight Interplay between Replication Stress and Competence Induction in

Cells. 2021-07; 
Vanessa Khemici, Marc Prudhomme, Patrice Polard
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Custom DNA/RNA Oligos … pneumoniae as defined by the OptimumGene™ algorithm and cloned into the pUC19 vector by Genscript USA to generate plasmid pUC19-I-sceI); second, a PCR fragment corresponding to CEP-P lac ::I-sceI (primers OVK301 and OVK304 using genomic DNA from R3313 strain… Get A Quote

摘要

Cells respond to genome damage by inducing restorative programs, typified by the SOS response of . (the pneumococcus), with no equivalent to the SOS system, induces the genetic program of competence in response to many types of stress, including genotoxic drugs. The pneumococcal competence regulon is controlled by the origin-proximal, auto-inducible CDE operon. It was previously proposed that replication stress induces competence through continued initiation of replication in cells with arrested forks, thereby increasing the relative CDE gene dosage and expression and accelerating the onset of competence. We have further investigated competence induction by genome stress. We find that absence of RecA recombina... More

关键词

DNA damage response, Streptococcus pneumoniae, bacterial competence, genome integrity, recombinational repair, replication stress