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Allelic diversification after transposable element exaptation promoted as the master sex determining gene of sablefish

Genome Res. 2021-06; 
Amaury Herpin, Manfred Schartl, Alexandra Depincé, Yann Guiguen, Julien Bobe, Aurélie Hua-Van, Edward S Hayman, Anna Octavera, Goro Yoshizaki, Krista M Nichols, Giles W Goetz, J Adam Luckenbach
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PCR Cloning and Subcloning … Subsequent 5′ and 3′ products were run on agarose gels, bands were punched and purified (Qiagen Gel Extraction Kit) and then cloned using the Zero Blunt TOPO PCR Cloning kit (Invitrogen) with at least three clones per amplicon sequenced in both directions (GenScript). … Get A Quote

摘要

Concepts of evolutionary biology suggest that morphological change may occur by rare punctual but rather large changes, or by more steady and gradual transformations. It can therefore be asked whether genetic changes underlying morphological, physiological, and/or behavioral innovations during evolution occur in a punctual manner, whereby a single mutational event has prominent phenotypic consequences, or if many consecutive alterations in the DNA over longer time periods lead to phenotypic divergence. In the marine teleost, sablefish (), complementary genomic and genetic studies led to the identification of a sex locus on the Y Chromosome. Further characterization of this locus resulted in identification of th... More

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