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GenParts™ DNA Fragments> | … DNA fragments were synthesized by GenScript and cloned into a bacterial expression vector for overexpression and purification (see below). Tetrahymena thermophila GLEBS domain was kept in the amino acid sequences for most of the FG domain variants because the … | Get A Quote |
The permeability barrier of nuclear pore complexes (NPCs) controls nucleocytoplasmic transport. It retains inert macromolecules while allowing facilitated passage of importins and exportins, which in turn shuttle cargo into or out of cell nuclei. The barrier can be described as a condensed phase assembled from cohesive FG repeat domains. NPCs contain several distinct FG domains, each comprising variable repeats. Nevertheless, we now found that sequence heterogeneity is no fundamental requirement for barrier function. Instead, we succeeded in engineering a perfectly repeated 12mer GLFG peptide that self-assembles into a barrier of exquisite transport selectivity and fast transport kinetics. This barrier recapitu... More