Products/Services Used | Details | Operation |
---|---|---|
Recombinant Antibody Expression> | … The fluorescently labelled auto-cleavage sequence of SARS-CoV-2 M pro , ((7-Methoxycoumarin-4-yl)acetyl)-AVLQ\(\downarrow \)SGFRK(2,4-dinitrophenyl)K (purchased from GenScript), was utilized to monitor the recombinant M pro kinetics (excitation 320 nm, emission 405 … | Get A Quote |
The maturation of coronavirus SARS-CoV-2, which is the etiological agent at the origin of the COVID-19 pandemic, requires a main protease M to cleave the virus-encoded polyproteins. Despite a wealth of experimental information already available, there is wide disagreement about the M monomer-dimer equilibrium dissociation constant. Since the functional unit of M is a homodimer, the detailed knowledge of the thermodynamics of this equilibrium is a key piece of information for possible therapeutic intervention, with small molecules interfering with dimerization being potential broad-spectrum antiviral drug leads. In the present study, we exploit Small Angle X-ray Scattering (SAXS) to investigate the structural fe... More