| Products/Services Used | Details | Operation |
|---|---|---|
| Plasmid DNA Preparation | … For the SWIRM 533–580 construct amino acids corresponding to that region of BAF155 were multimerized four times in a plasmid containing the appropriate restriction enzyme sites (GenScript) and inserted in pSUMO. All SUMO constructs including SUMO alone were … | Get A Quote |
The SNF5 subunit of the SWI/SNF chromatin remodeling complex has been shown to act as a tumor suppressor through multiple mechanisms, including impairing the ability of the oncoprotein transcription factor MYC to bind chromatin. Beyond SNF5, however, it is unknown to what extent MYC can access additional SWI/SNF subunits or how these interactions affect the ability of MYC to drive transcription, particularly in SNF5-null cancers. Here, we report that MYC interacts with multiple SWI/SNF components independent of SNF5. We show that MYC binds the pan-SWI/SNF subunit BAF155 through the BAF155 SWIRM domain, an interaction that is inhibited by the presence of SNF5. In SNF5-null cells, MYC binds with remaining SWI/SNF... More
