| Products/Services Used | Details | Operation |
|---|---|---|
| DNA Sequencing | … After murinization and cysteine modification of the constant domains of the TCR and insertion of an additional cysteine bridge, the complete sequence of both TCR chains was constructed with a 2A sequence for bicistronic expression (GenScript, Piscataway Township, NJ, USA) … | Get A Quote |
Human leukocyte antigen-E (HLA-E) normally presents an HLA class Ia signal peptide to the NKG2A/C-CD94 regulatory receptors on natural killer (NK) cells and T cell subsets. Rhesus macaques immunized with a cytomegalovirus-vectored simian immunodeficiency virus (SIV) vaccine generated Mamu-E (HLA-E homolog)-restricted T cell responses that mediated post-challenge SIV replication arrest in >50% of animals. However, HIV-1-specific, HLA-E-restricted T cells have not been observed in HIV-1-infected individuals. Here, HLA-E-restricted, HIV-1-specific CD8 T cells were primed in vitro. These T cell clones and allogeneic CD8 T cells transduced with their T cell receptors suppressed HIV-1 replication in CD4 T cells in... More
