Products/Services Used | Details | Operation |
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ORF cDNA Clones/MolecularCloud> | … cDNAs encoding for the L and NP ORFs as well as for the full-length L and S segments of CAND and PICV were synthesized by Genscript, the Netherlands, and were ligated into polymerase-II- and polymerase-I-driven expression cassettes, respectively. MOPV cDNAs for virus … | Get A Quote |
Therapeutic vaccination regimens inducing clinically effective tumor-specific CD8 T lymphocyte (CTL) responses are an unmet medical need. We engineer two distantly related arenaviruses, Pichinde virus and lymphocytic choriomeningitis virus, for therapeutic cancer vaccination. In mice, life-replicating vector formats of these two viruses delivering a self-antigen in a heterologous prime-boost regimen induce tumor-specific CTL responses up to 50% of the circulating CD8 T cell pool. This CTL attack eliminates established solid tumors in a significant proportion of animals, accompanied by protection against tumor rechallenge. The magnitude of CTL responses is alarmin driven and requires combining two genealogicall... More