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Basigin drives intracellular accumulation of l-lactate by harvesting protons and substrate anions

PLoS ONE. 2021-03; 
Anna-Lena Köpnick, Annika Jansen, Katharina Geistlinger, Nathan Hugo Epalle, Eric Beitz
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摘要

Transmembrane transport of l-lactate by members of the monocarboxylate transporter family, MCT, is vital in human physiology and a malignancy factor in cancer. Interaction with an accessory protein, typically basigin, is required to deliver the MCT to the plasma membrane. It is unknown whether basigin additionally exerts direct effects on the transmembrane l-lactate transport of MCT1. Here, we show that the presence of basigin leads to an intracellular accumulation of l-lactate 4.5-fold above the substrate/proton concentrations provided by the external buffer. Using basigin truncations we localized the effect to arise from the extracellular Ig-I domain. Identification of surface patches of condensed opposite el... More

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